Human Ornithine carbamoyltransferase, mitochondrial (OTC) ELISA Kit

This Sandwich ELISA kit from Assay Genie is designed for the quantitative detection of human Ornithine carbamoyltransferase, mitochondrial (OTC), also known as ornithine transcarbamylase or OTCase. The assay uses a colorimetric detection method and is reactive with human samples, including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

The kit provides a detection range of 3.12-200 U/L and a sensitivity of 1.5 U/L. Reported precision is 4.6% intra-assay CV and 8.9% inter-assay CV. In dilution linearity testing, serum samples (n=5) returned 89-99% at 1:2, 107-117% at 1:4, 93-102% at 1:8 and 98-106% at 1:16, with EDTA plasma ranging 89-117% and heparin plasma 81-117% across the same dilutions. Spike-and-recovery was 100% (range 94-106%) for serum and 102% (range 96-108%) for plasma. The assay is specific to natural and recombinant human OTC and is intended for research use only.

OTC is a mitochondrial matrix enzyme (Uniprot P00480; EC 2.1.3.3; homotrimer) that catalyses a key step in the urea cycle. The kit from Assay Genie is suited to studies of urea cycle disorders, including ornithine carbamoyltransferase deficiency — an X-linked condition (Xp21.1) that can lead to hyperammonemia — as well as liver and metabolic disease research.