Human Ornithine Carbamoyl Transferase (OCT) ELISA Kit

Mitochondrial ammonia disposal in hepatocytes depends on the urea cycle enzyme Ornithine Carbamoyl Transferase, whose deficiency underlies OTCD, the most common inherited urea cycle disorder. Quantitative measurement of this enzyme in research samples supports studies of hyperammonemia, hepatic injury, mitochondrial dysfunction, and inherited metabolic disease.

Assay Genie supplies this human OCT ELISA kit in a 96-assay microplate configuration with colorimetric readout. The assay is validated for human samples, including tissue homogenates and blood-derived specimen types, making it applicable to liver biopsy material, hepatic cell line lysates, and patient-derived specimens in research workflows.

Each kit provides a pre-coated microplate, reference standards, detection antibody, and substrate reagents sufficient for 96 assays. The workflow follows standard immunoassay principles: sample addition, incubation, washing, conjugate binding, and absorbance measurement at an appropriate wavelength.

Research use only. Allow reagents to reach room temperature before use, and minimize freeze-thaw cycles of prepared samples to preserve antigen integrity. Protect light-sensitive components from prolonged exposure.