Human OGT ELISA Kit (HUFI07423)

The OGT enzyme, also known as O-linked N-acetylglucosamine (GlcNAc) transferase, catalyzes the addition of O-GlcNAc modifications to serine and threonine residues of target proteins. This post-translational modification is central to insulin signaling, transcription regulation, epigenetic control, and cellular stress response, with dysregulated OGT activity implicated in type 2 diabetes, Alzheimer's disease, and tumor progression.

Researchers apply this Assay Genie kit to quantify circulating OGT protein in human-derived samples, supporting biomarker discovery, hexosamine biosynthesis pathway studies, and chromatin remodeling investigations. The microplate kit includes pre-coated wells, biotinylated detection antibody, HRP-streptavidin conjugate, and lyophilized recombinant standards calibrated for accurate measurement. A typical protocol uses 100 uL of sample per well, with absorbance readout enabling quantitative comparison across experimental cohorts. Reagents should be stored refrigerated between uses and protected from light to preserve antibody activity through the workflow.