Human ODC/Ornithine decarboxylase ELISA Kit

From Assay Genie, this high-quality sandwich ELISA kit is designed for the accurate detection of Ornithine Decarboxylase (ODC) levels in human samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a colorimetric detection method with a double-antibody sandwich format, offering high sensitivity and specificity for reliable and reproducible results across a variety of research applications.

ODC is a key enzyme involved in polyamine biosynthesis, playing a critical role in cell growth and proliferation. Abnormal ODC levels have been linked to diseases such as cancer, inflammatory conditions, and neurological disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions. The kit delivers a sensitivity of 0.188 ng/mL with a measurable range of 0.313–20 ng/mL, and reagents remain stable for 12 months when stored at 2–8°C.

The workflow follows a standard sandwich ELISA procedure: equilibrate reagents and TMB substrate to room temperature, incubate standards and samples at 37 °C to allow antigen binding, add a biotin-labelled detection antibody followed by HRP-Streptavidin, develop colour with TMB substrate in the dark, then stop the reaction and read absorbance at 450 nm. Linearity and recovery have been validated across serum, EDTA plasma, and heparin plasma.