Human OCRL ELISA Kit (HUFI07429)

OCRL is an inositol polyphosphate-5-phosphatase localized primarily to the trans-Golgi network and early endosomes, where it hydrolyzes phosphatidylinositol 4,5-bisphosphate to regulate membrane trafficking and clathrin-mediated endocytosis. Loss-of-function mutations in OCRL cause Lowe syndrome (oculocerebrorenal syndrome) and Dent disease type 2, making quantitative protein measurement valuable for research into these rare inherited disorders.

This immunoassay kit from Assay Genie is configured as a 96 Assays microplate format and supplies all components required for routine laboratory immunoassay workflows, including pre-coated wells, a calibrated reference standard, biotinylated detection reagent, streptavidin-HRP conjugate, chromogenic substrate solution, wash buffer, and stop reagent. Standard handling protocols recommend refrigerated storage of unopened components to preserve antibody binding activity and substrate integrity until first use.

Researchers investigating phosphoinositide signaling pathways, Golgi-endosomal trafficking defects, or biomarker discovery in patient-derived liquid specimens can incorporate this assay into broader panel designs alongside complementary cytokine or kidney-injury markers. Each lot is quality-controlled for inter- and intra-assay precision, and the protocol supports up to 96 individual determinations when standards and unknowns are run in duplicate or triplicate. Intended for research use only.