Human OCLN/Occludin ELISA Kit

For the quantification of human occludin (OCLN), this Assay Genie Sandwich ELISA Kit employs a colorimetric detection method and is validated for serum and plasma samples. The kit supports research into tight junction biology, where occludin contributes to epithelial and endothelial barrier integrity.

Manufacturer-reported specifications for the Sandwich ELISA, Double Antibody format indicate a sensitivity of 18.75 pg/mL and a working range of 31.25-2000 pg/mL. Linearity across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions falls between 80% and 105%. Average recovery is 93% (serum), 96% (EDTA plasma), and 91% (heparin plasma). The procedure includes a 37°C primary incubation of 90 minutes, biotinylated detection antibody binding, HRP-streptavidin binding, TMB substrate development, and absorbance measurement at 450 nm. Components are stored at 2-8°C for 12 months.

Dysregulation of occludin has been associated with inflammatory bowel disease, coeliac disease, and certain cancers, supporting applications in disease pathogenesis research and therapeutic target identification.