Human OCEL1 (Occludin/ELL domain-containing protein 1) ELISA KIT (HUFI05002)

OCEL1 carries both an occludin domain and an ELL (Eleven-nineteen Lysine-rich Leukemia) domain, a dual architecture that places this protein at the intersection of tight-junction biology and transcription elongation research. Quantifying OCEL1 therefore supports studies of epithelial barrier integrity, cell-cell adhesion dynamics, and nuclear transcriptional processes in human cellular systems.

This Assay Genie kit is supplied with a pre-coated capture microplate, lyophilised recombinant OCEL1 standards, a biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, concentrated wash buffer, and adhesive plate sealers. The kit supports 96 individual reactions, and absorbance is read on a standard microplate reader following the included step-by-step protocol.

Samples collected from human subjects should be processed using standard separation procedures to yield cell-free liquid fractions appropriate for the assay. Reconstituted standards and concentrated detection reagents benefit from being aliquoted into single-use vials and stored frozen to limit repeated freeze-thaw cycles, while the pre-coated microplate is kept refrigerated until use.

Intended for research use only. Investigators apply this kit to biomarker discovery, comparative expression profiling across tissue contexts, and characterisation of barrier-related pathophysiology in human cohorts.