Human OBSCN ELISA Kit (HUFI07427)

Built by Assay Genie for quantifying the OBSCN gene product in human samples, this ELISA kit targets obscurin, a giant striated-muscle scaffold protein with an apparent molecular mass of approximately 800 kDa. The analyte localizes to sarcomeric M-bands and Z-discs, where it links contractile filaments to the sarcoplasmic reticulum and contributes to myofibril assembly and intracellular signaling.

Researchers in cardiac and skeletal muscle biology apply the assay to studies of dilated cardiomyopathy, hypertrophic cardiomyopathy, M-line organization, and titin-related mechanotransduction. The kit is supplied as a 96-assay microplate format with the reagents, plates, and buffers needed to complete one full-plate run, and is intended for research use only.

Obscurin expression is altered in several inherited and acquired muscle disorders, including cardiomyopathies associated with truncating OBSCN variants and structural remodeling after myocardial injury. The kit supports quantitative readouts from a standard curve generated with the supplied calibrator, allowing interpolation of obscurin concentrations across matched study samples. Validated sample types include serum-derived and plasma-derived preparations from human donors, and protocols are organized around a routine plate-based immunoassay workflow.