Human OBFC2B ELISA Kit (HUFI07426)

The OBFC2B target, also known as SOSSB2 or Sensor of Single-Stranded DNA Complex Subunit B2, encodes an oligonucleotide/oligosaccharide-binding (OB) fold protein that functions as a component of the SOSS1 single-strand DNA sensor complex. The SOSS1 complex recognizes exposed single-stranded DNA at sites of DNA double-strand breaks and contributes to ATM-dependent checkpoint signaling, homologous recombination repair, and the maintenance of telomere integrity. Researchers studying genotoxic stress responses, replication fork collapse, or telomere dysfunction rely on quantitative measurement of OBFC2B protein levels in patient and experimental samples.

This quantitative immunoassay from Assay Genie supports studies where OBFC2B abundance is being evaluated in clinical and research specimens. The kit ships as a microplate-format package with pre-coated wells, biotinylated detection antibody, recombinant standards, and substrate reagents, allowing laboratories to generate a standard curve and quantify native OBFC2B in their preparations. The assay uses a human-specific capture antibody pair. Each well requires a 100 uL sample volume, and the protocol is performed in vitro using standard laboratory ELISA plate readers. Investigators in oncology, radiation biology, and DNA repair fields apply this measurement to correlate OBFC2B levels with disease stage, treatment response, or genotype. For research use only; not intended for diagnostic procedures.