Human Nucleoside phosphorylase ELISA Kit (HUFI07455)

Purine nucleoside phosphorylase (PNP, EC 2.4.2.1) is a key enzyme of the purine salvage pathway, catalyzing the reversible phosphorolysis of inosine and guanosine to the corresponding free bases and ribose-1-phosphate. Quantification of circulating PNP by ELISA supports investigations into severe combined immunodeficiency (SCID), T-cell maturation defects, autoimmune disorders, and hematological cancers such as T-cell acute lymphoblastic leukemia.

The Human Nucleoside phosphorylase ELISA Kit from Assay Genie is configured for 96 Assays and is specific for the human enzyme with no significant cross-reactivity with rodent PNP. The kit ships with a pre-coated microplate, biotinylated detection antibody, lyophilized standards, streptavidin-HRP conjugate, and substrate solution. Researchers often pair this colorimetric read-out with Western blot or enzymatic activity assays to confirm functional deficits in patient samples.