Human Nucleoredoxin (NXN) ELISA Kit

The Assay Genie Human Nucleoredoxin (NXN) ELISA Kit enables quantitative measurement of nucleoredoxin, a nuclear-localized thioredoxin-family oxidoreductase that acts as a negative regulator of the Wnt/β-catenin signaling pathway through binding of Dishevelled (Dvl) proteins. This two-antibody immunoassay employs a human-specific capture antibody and is validated for quantitative detection in tissue homogenates and cell lysate-derived samples. Researchers studying developmental biology, redox homeostasis, or Wnt-pathway-related disease models can use the assay to measure endogenous NXN protein levels. Reconstituted standards and detection antibody should be stored frozen at -20°C, while unused microplate strip wells remain stable sealed in their foil pouch with desiccant under refrigerated conditions. The kit includes a pre-coated microplate, biotin-conjugated detection reagent, and streptavidin-HRP working solution for research use only.