Human Nucleophosmin (NPM) ELISA Kit

Nucleophosmin (NPM1) is a nucleolar phosphoprotein involved in ribosome biogenesis, centrosome duplication, and maintenance of genomic integrity. Mutations and overexpression of NPM1 are recurrent events in hematological cancers, with NPM1 alterations observed in roughly 30% of adult acute myeloid leukemia (AML) cases. This quantitative immunoassay kit from Assay Genie is configured for human reactivity and supplied in a 96-assay microplate format with all reagents required to measure NPM abundance in research samples. Validated sample matrices include tissue homogenates and cell lysates, supporting workflows in leukemia cell line characterization, tumor tissue profiling, and studies of nucleolar stress responses. The assay uses an absorbance-based readout compatible with standard plate readers, allowing laboratories to incorporate NPM quantification into existing in vitro screening pipelines. Researchers working on AML pathogenesis, nucleolar biology, and oncogenic signaling can apply this kit to monitor expression changes, compare NPM levels across patient-derived and model samples, and investigate drug-induced nucleolar disruption. Each kit ships with a pre-coated plate and is intended for research use only, providing a practical tool for studies aimed at biomarker discovery and therapeutic response profiling.