Human Nucleolar GTP-binding protein 1 (GTPBP4) ELISA Kit

Assay Genie supplies a Sandwich ELISA kit for the quantitative measurement of Human Nucleolar GTP-binding protein 1 (GTPBP4) in research samples. The colourimetric assay uses a microtiter plate pre-coated with a specific capture antibody, combined with a biotinylated detection antibody and an Avidin-Horseradish Peroxidase (HRP) conjugate; the enzymatic reaction is terminated and the optical density is read at 450 nm.

Performance figures quoted by the manufacturer are a detection range of 0.312-20ng/mL and a sensitivity of 0.164ng/mL. The kit is reactive with human samples and is validated for serum, plasma, saliva, urine, cell culture supernatant, tissue homogenates, and other related biological fluids. Specificity covers natural and recombinant human Nucleolar GTP-binding protein 1. Listed synonyms include Chronic renal failure gene protein, GTP-binding protein NGB, CRFG, and NOG1. The research area is Epigenetics, the target localises to the nucleus and nucleolus (UniProt Q9BZE4), and the protein is involved in the biogenesis of the 60S ribosomal subunit. Dysregulation of GTPBP4 has been linked to cancer and neurodevelopmental disorders, highlighting its importance as a potential biomarker for disease research and therapeutic development.

The kit is for research use only and is compatible with routine laboratory and high-throughput screening (HTS) formats. It is shipped at +4°C; the assay plate, standard, and detection reagents are stored at -20°C, with the substrate held at 4°C. The kit is assigned an expiration date of six months from the date of manufacture and remains stable until that date when stored as prescribed.