Human Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) ELISA Kit

Rev-erbβ (Nuclear Receptor Subfamily 1, Group D, Member 2, or NR1D2) is a heme-binding transcriptional repressor that operates through ROR response elements to regulate circadian rhythm, lipid metabolism, and inflammatory gene expression. The Human NR1D2 ELISA Kit from Assay Genie provides a quantitative two-antibody immunoassay for measuring NR1D2 protein levels in human tissue homogenates and other biological sample matrices.

Each kit supplies reagents for 96 individual reactions on a pre-coated microplate, accommodating both small mechanistic experiments and larger cohort screens in chronobiology, nuclear receptor biology, and metabolic disease research. Investigators studying hepatic, adipose, neural, and immune tissues use this assay to track NR1D2 dynamics across varying physiological and pharmacological conditions.

Detection relies on a colorimetric readout compatible with standard plate readers, producing reproducible absorbance measurements across a standard curve. The workflow incorporates ready-to-use standards, detection antibody, and wash components to streamline laboratory use. Storage recommendations in the product insert should be followed to preserve reagent activity through the kit's stated shelf life.

For research use only, this assay is validated for human samples and supports reproducible protein quantification in studies ranging from in vitro cell culture work to in vivo experimental models.