Human Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) ELISA Kit

For researchers investigating cellular defense mechanisms against oxidative damage, this quantitative immunoassay provides a reliable means of measuring NFE2L2, the protein widely known as Nrf2. Nrf2 acts as the master regulator of the antioxidant response, coordinating transcription of phase II detoxifying enzymes and cytoprotective proteins through antioxidant response elements. Dysregulated Nrf2 signaling has been implicated in cancer progression, neurodegenerative disorders, inflammatory conditions, and chemical toxicity, making accurate quantification a priority across multiple research fields.

Manufactured by Assay Genie, the kit is supplied as a complete reagent set sufficient for 96 individual determinations. It supports quantification of endogenous human NFE2L2 in tissue homogenates and cell lysates prepared from cultured cells or experimental animal models. The procedure follows a standard immunometric workflow in which captured analyte is detected via an enzyme-linked secondary antibody, with absorbance readout on a standard microplate reader. All components are intended for research use only and are not approved for diagnostic procedures.

Application areas include screening of pharmacological compounds that modulate the Nrf2/Keap1 pathway, monitoring Nrf2 activation in response to electrophilic stress or chemopreventive agents, and comparative studies of Nrf2 expression between healthy and diseased tissues. The kit pairs well with downstream western blot validation and qPCR analysis of Nrf2 target genes to provide complementary protein-level and transcript-level readouts.