Human NT5M ELISA Kit (HUFI07471)

NT5M encodes the mitochondrial 5'(3')-nucleotidase, an enzyme localized to the mitochondrial matrix that hydrolyzes nucleoside monophosphates and supports nucleotide salvage. Researchers studying mitochondrial metabolism, mtDNA repair, or disorders of cellular energetics can use this kit to quantify NT5M levels.

The Assay Genie microplate format provides 96 Assays, allowing duplicate measurements of standards and samples across a full standard curve. The procedure is compatible with routine laboratory workflows and can be paired with downstream methods such as western blot or PCR for validation of expression changes.

Reagents should be stored refrigerated at 4 degrees C, with frozen aliquots kept at -20 degrees C for long-term stability. Vials must be protected from light and allowed to equilibrate to room temperature before each run. Avoid excessive freeze-thaw cycles to preserve antibody binding activity.