Human NT-proBNP (NT-proBNP) ELISA Kit

From Assay Genie, this Sandwich ELISA kit enables quantitative detection of human NT-proBNP (N-terminal prohormone of brain natriuretic peptide) using a colorimetric ELISA method. Validated sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, with reactivity confirmed for human samples.

Reported performance characteristics include a detection range of 0.312–20 ng/mL and a sensitivity of 0.12 ng/mL. Precision is given as 5.9% intra-assay CV and 7.8% inter-assay CV. Dilutional linearity across 1:2 to 1:16 dilutions ranged from 88–124% in serum, 80–120% in EDTA plasma, and 84–109% in heparin plasma. Recovery averaged 97% (range 91–103) for serum and 99% (range 93–105) for plasma. NT-proBNP is a 76 amino acid N-terminal fragment of brain natriuretic peptide and a recognised biomarker for cardiac stress, used in cardiovascular research on conditions such as heart failure and myocardial infarction. Specificity is reported for natural and recombinant human NT-proBNP (UniProt reference NP_002512.1).