Human NRN1L (Neuritin-like protein) ELISA KIT (HUFI05022)

Neuritin-like protein (NRN1L) is a GPI-anchored member of the neuritin family implicated in neurite outgrowth, dendritic spine maturation, and activity-dependent synaptic plasticity. This ELISA Kit from Assay Genie provides quantitative measurement of NRN1L in human research samples, supporting neuroscience investigations into neural development, plasticity, and emerging oncology studies where NRN1L has been explored as a candidate biomarker.

The kit is configured for a 96 Assays workflow using a two-antibody sandwich format on a pre-coated microplate. A colorimetric enzyme-substrate reaction produces a signal proportional to NRN1L concentration, read on a standard microplate reader. Contents include the capture plate, lyophilized recombinant protein standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, assay diluent, chromogenic substrate, and stop solution, supplied in single-use vial and well plate formats. The kit can be applied to serum or EDTA-plasma matrices from human donors under research-use protocols.

Workflow steps include preparing a standard dilution series, loading controls and samples onto the plate, completing sequential incubation and wash cycles, adding substrate for chromogenic development, stopping the reaction, and reading absorbance. Reagents should be equilibrated to room temperature before use; kit components are stored refrigerated at manufacturer-recommended temperatures and protected from light to maintain activity across the shelf life of the product.