Human Notch-3 PharmaGenie ELISA Kit (SBRS0935)

Notch-3 is a single-pass transmembrane receptor that mediates cell-fate decisions and is required for proper vascular smooth muscle maturation, with NOTCH3 mutations linked to CADASIL, a hereditary small-vessel disease. The Assay Genie Human Notch-3 PharmaGenie ELISA Kit provides quantitative measurement of this protein in cell culture supernatant, plasma, or serum, supporting vascular biology and translational neurology research.

Each kit contains reagents sufficient for 96 assays in a strip plate format compatible with standard microplate readers. The absorbance-based detection reaction is read at a wavelength suited for routine plate-reader optics, and the assay is calibrated specifically for human samples. Researchers use it to quantify the shed Notch-3 ectodomain in conditioned media from cultured cells, paired plasma, or matched serum from clinical cohorts.

For stability, store the sealed kit refrigerated between uses; cold storage preserves the binding activity of capture and detection antibodies throughout the stated shelf life. The kit is supplied with a pre-coated microplate, assay diluent, wash buffer, standards, and detection antibody, and is intended for research use only — not for diagnostic procedures.