Human Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) ELISA Kit

The NME1 protein, also known as NM23A or nucleoside diphosphate kinase A, belongs to a conserved family of multifunctional enzymes first characterized through reduced expression in highly metastatic melanoma cell lines. Beyond its classical role in nucleoside triphosphate synthesis, NME1 exhibits histidine kinase activity, contributes to DNA repair and apoptosis regulation, and functions as a transcriptional activator for specific target genes, making it a subject of broad interest in cancer biology and cellular signaling research.

Loss of NME1 activity has been documented in aggressive breast, lung, hepatocellular, and colorectal tumors, while retained or elevated expression can correlate with favorable prognosis in selected patient cohorts. This Assay Genie kit provides quantitative measurement of human NME1 in serum specimens, plasma preparations, tissue homogenates, and cultured cell lysates, using an immunoassay on a 96-assay microplate with a colorimetric endpoint readable on standard laboratory plate readers. Each plate arrives pre-coated with capture antibody, and the kit supplies biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer, substrate solution, and a recombinant NME1 standard for calibration curve generation.

Researchers deploy this format to compare protein levels across case and control populations, monitor tumor progression in animal models, or quantify cellular responses to candidate therapeutics during cell culture experiments. Application areas include cancer metastasis research, characterization of the NME1 signaling network and its interactions with the Ras and ERK pathways, and studies of nucleotide metabolism and cellular differentiation. The kit is intended for research use only by trained laboratory personnel conducting in vitro analyses.