Human Nogo-B/RTN 4B PharmaGenie ELISA Kit (SBRS0931)

This quantitative immunoassay from Assay Genie measures human Nogo-B, the shorter reticulon isoform known as RTN4B and encoded by the RTN4 gene. Nogo-B is a transmembrane endoplasmic reticulum protein implicated in vascular remodeling, macrophage migration, and apoptotic signaling, making it an active target in cardiovascular research, oncology, and inflammation studies.

The kit provides a complete reagent set for use on a standard microplate, with pre-coated capture antibodies and a biotinylated detection antibody paired with a streptavidin-enriched reporter. Researchers can quantify Nogo-B in clarified cell culture supernatant, heparinized plasma, and serum specimens using a 100 µL sample volume per well. The assay's capture antibody is human-specific for RTN4B, with no significant cross-reactivity observed against rodent reticulon isoforms.

Store all components refrigerated at 4 °C upon arrival, and limit reconstituted standards and prepared samples to no more than two freeze-thaw cycles. Each kit is shipped in a sealed foil pouch containing the antibody-coated well plate, lyophilized standard, detection antibody vial, wash buffer concentrate, substrate solution, and stop reagent.