Human NODAL/Nodal homolog ELISA Kit

From Assay Genie, this Sandwich ELISA kit is configured for the quantitative measurement of human Nodal (Nodal homolog) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. It uses a double-antibody sandwich format with colorimetric detection. Nodal is described in the product documentation as a critical protein involved in embryonic development, stem cell maintenance, and cancer progression, with dysregulation linked to various diseases and conditions, offering researchers a reliable method to investigate the role of Nodal in health and disease.

Reported performance includes a sensitivity of 46.875 pg/mL and a standard range of 78.125-5000 pg/mL. Validation across serum, EDTA plasma, and heparin plasma (n=5) shows an average recovery of 96% per matrix (recovery ranges 85-103% across matrices) and linearity of 80-105% at sample dilutions of 1:2, 1:4, and 1:8. The kit's associated UniProt reference is Q96S42 and the listed research area is cell biology. Components are stored at 2-8°C for up to 12 months, with several reagents specified to be protected from direct light.

The assay workflow equilibrates reagents and TMB substrate to room temperature before loading standards, samples, and controls on the pre-coated plate. The plate is incubated at 37°C for 90 minutes for antigen binding, followed by 60 minutes with a biotin-labeled detection antibody at 37°C, then 30 minutes with HRP-Streptavidin (SABC) at 37°C. TMB substrate is added and incubated in the dark for 10-20 minutes; after stop solution, absorbance is read at 450 nm.