Human NO66/C14orf169 ELISA Kit (HUFI07495)

Quantitative measurement of the NO66 histone demethylase in human research specimens is achieved through this microplate immunoassay from Assay Genie, supporting studies of chromatin modification and ribosome biogenesis. NO66, encoded by the chromosome 14 open reading frame 169 gene, is a JmjC-domain oxygenase that catalyzes oxidative demethylation of histone H3 lysine residues and localizes to the nucleolus. Investigators working in epigenetic regulation, ribosomal RNA processing, or osteogenic differentiation pathways use the kit to monitor NO66 protein abundance across experimental conditions.

Each kit is supplied as a complete reagent set sufficient for ninety-six determinations on a standard microplate, with coated wells, calibrated protein standards, detection antibody, substrate solution, and assay buffer. The workflow accommodates batch processing of study samples, supporting in vitro investigation of chromatin-modifying enzyme dynamics in cell culture models and translational research cohorts. The assay is intended for research use only and has not been validated for diagnostic procedures.

For optimal reagent stability, reconstituted components should be stored refrigerated and protected from light. Allow all components to equilibrate to room temperature before initiating the protocol, and limit repeated freeze-thaw cycles of the standard and detection antibody. Standard curves generated from serial dilutions enable interpolation of NO66 concentrations in unknown samples, facilitating comparative analyses between treated and control groups in stem cell, bone biology, and chromatin research programs.