Human NMNAT-1 PharmaGenie ELISA Kit (SBRS0928)

The Human NMNAT-1 target, Nicotinamide Mononucleotide Adenylyltransferase 1, is the nuclear isoform of the enzyme that catalyses the final step of the NAD⁺ salvage biosynthesis pathway, converting NMN and ATP into NAD⁺. This Assay Genie PharmaGenie reagent enables quantitative measurement of the human protein and is intended for in vitro research use only.

NMNAT-1 sits at the centre of investigations into cellular energy homeostasis, mitochondrial-to-nuclear NAD⁺ trafficking, and axon survival in the Wallerian degeneration slow (Wld^S) model. Loss-of-function mutations are causally linked to Leber Congenital Amaurosis type 9 (LCA9) and other rare neurodevelopmental disorders, making accurate quantification of the human protein valuable for translational ophthalmology, neuroscience, and metabolism research.

Workflow: reconstitute reagents per the lot-specific manual, add 100 µL of calibrator or prepared sample to each well of the pre-coated plate, perform the prescribed incubation and wash steps, develop with TMB substrate, and read absorbance to interpolate concentration against the standard curve. Store unopened kit components refrigerated at 4 °C and keep the substrate vial protected from light; reconstituted reagents and residual samples should be aliquoted and held at -20 °C to limit freeze-thaw cycles.