Human NMDAR2A/GRIN2A ELISA Kit

Designed for the quantitative measurement of NMDAR2A (GRIN2A) protein in human samples, this sandwich ELISA from Assay Genie targets a critical subunit of the N-methyl-D-aspartate (NMDA) receptor, which is essential for neurological functions including learning and memory. Dysregulation of NMDAR2A has been linked to conditions such as schizophrenia, epilepsy, and Alzheimer's disease, making it a relevant biomarker for studying neurological disorders.

The assay is suitable for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with reactivity confirmed for human specimens. It employs a double-antibody sandwich format with colorimetric detection. Performance characteristics include a sensitivity of 0.188 ng/mL and a measuring range of 0.313-20 ng/mL. Linearity has been validated across dilution ratios of 1:2, 1:4, and 1:8 in serum (82-98%, 86-100%, 81-95%), EDTA plasma (90-98%, 85-101%, 85-91%), and heparin plasma (88-100%, 82-96%, 86-97%). Spike-and-recovery values are reported as 87-102% (serum), 89-104% (EDTA plasma), and 87-101% (heparin plasma), with averages of 95%, 99%, and 98% respectively.

The protocol involves equilibrating reagents, primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody incubation for 60 minutes, HRP-streptavidin binding for 30 minutes, TMB colour development in the dark for 10–20 minutes, and absorbance reading at 450 nm. Reagents should be stored at 2-8°C, where the kit remains stable for 12 months. The target protein is catalogued under UniProt ID Q12879 and KEGG pathway K05209.