Human NLRP3 (Nod Like Receptor Pyrins-3) ELISA Kit (HUFI04745)

NLRP3 (NOD-like receptor pyrin domain-containing protein 3) functions as the sensor subunit of the NLRP3 inflammasome, a cytosolic signaling platform that activates caspase-1 and triggers the maturation and secretion of IL-1β and IL-18. Dysregulated NLRP3 activity is associated with cryopyrin-associated periodic syndromes (CAPS), gout, atherosclerosis, type 2 diabetes, non-alcoholic steatohepatitis, and several neurodegenerative conditions, which has driven demand for reliable protein-level quantification in cell lysates, tissue homogenates, and biofluids.

Researchers using this Assay Genie reagent pair the kit with matched sample collection protocols; cells are typically lysed in RIPA buffer supplemented with protease inhibitors, and blood-derived specimens are processed using conventional venipuncture and centrifugation workflows. The microplate is pre-coated with a capture antibody specific to human NLRP3, and detection proceeds through a biotinylated secondary antibody and streptavidin-conjugated reporter. Reconstituted standards should be aliquoted into single-use vials to minimize repeated freeze-thaw cycles, and the working conjugate should be brought to room temperature immediately before use.

Intended for research use only, the assay is compatible with standard absorbance plate readers and can be integrated into studies of innate immune signaling, drug screening for inflammasome inhibitors, and biomarker development for inflammatory disease cohorts.