Human NLR family CARD domain-containing protein 4 (NLRC4) ELISA Kit

A sandwich ELISA from Assay Genie for the quantitative detection of human NLR family CARD domain-containing protein 4 (NLRC4). The assay uses a colorimetric detection method and is reactive on human samples including serum, plasma, tissue homogenates, and cell culture supernates. According to the manufacturer, NLRC4 functions as a key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi, responding by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production, and macrophage pyroptosis. The assay is described as specific for natural and recombinant human NLRC4 and is supplied for research use only.

The reported detection range is 78–5000 pg/mL with a sensitivity of 39 pg/mL. Intra-assay precision is reported as 4.3% CV and inter-assay precision as 8.2% CV. The kit's listed research area is cancer, reflecting NLRC4's role in apoptosis and inflammation. Dysregulation of NLRC4 has been implicated in inflammatory diseases, autoimmune disorders, and infectious conditions, making the protein a useful biomarker for studies of innate immunity and immune system regulation.