Human NLGN4X ELISA Kit

Assay Genie offers a Sandwich ELISA, Double Antibody kit designed for the quantitative measurement of human NLGN4X in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay is colorimetric and uses a pre-coated plate format: standards and samples are incubated at 37°C to allow antigen binding, followed by detection with a biotin-labeled antibody, HRP-Streptavidin, and TMB substrate, with absorbance read at 450 nm.

Reported analytical performance includes a sensitivity of 0.188 ng/mL and a measuring range of 0.313-20 ng/mL. Kit components are stored at 2-8°C (with light-sensitive items such as the TMB substrate stored away from direct light) and are stated to remain stable for 12 months under these conditions. NLGN4X is described as a protein involved in synaptic function and brain development, with mutations in the NLGN4X gene linked to neurodevelopmental disorders such as autism spectrum disorders, making the kit a tool for research into neurological disorders and synaptic function.