Human Nitric Oxide Synthase 1, Neuronal (NOS1) ELISA Kit

Researchers studying neuronal nitric oxide signaling rely on quantitative ELISA-based immunoassays to measure Nitric Oxide Synthase 1 (nNOS) levels across experimental and clinical cohorts. This Assay Genie kit provides a 96-assay plate-based workflow for quantifying the ~161 kDa human enzyme, supporting laboratory research in neuroscience, vascular biology, and inflammation pathways.

The colorimetric method produces an absorbance readout proportional to nNOS concentration in each specimen. Validated reactivity is restricted to human samples, with the kit accommodating serum-derived specimens, plasma-derived specimens, and tissue homogenate preparations. Reagents require refrigerated storage and standards should be kept on ice during use; repeated freeze-thaw cycles of diluted standards should be avoided to preserve antibody binding activity.

Each kit contains pre-coated microplate wells, biotinylated detection reagent, a standard curve set, and assay buffers sufficient for 96 individual determinations. The workflow involves sequential incubation, washing, and signal development steps compatible with standard automated plate washers and multichannel pipettes, enabling batch processing of patient cohorts or experimental samples.

Applications include monitoring nNOS expression in neurodegenerative disease models, characterizing nitric oxide pathway activity in cardiovascular research, and investigating neuronal nitric oxide synthase contributions to inflammatory pathophysiology. The kit is validated for human samples; investigators working with other species should verify cross-reactivity independently before drawing quantitative conclusions.