Human Nidogen-1 PharmaGenie ELISA Kit (SBRS0923)

Nidogen-1 (also called entactin) is a sulfated basement membrane glycoprotein that links laminin and collagen IV networks within the extracellular matrix, supporting cell adhesion and tissue architecture. This PharmaGenie ELISA Kit from Assay Genie quantifies human Nidogen-1 levels across sample matrices including conditioned media from cultured cells, plasma-derived fractions, and matched serum aliquots, making it suitable for studies of basement membrane remodeling, fibrosis research, and tumor microenvironment composition. The kit includes a pre-coated microplate and all reagents required for an absorbance-based readout on a standard plate reader. The workflow provides ready-to-use standards and buffers for standard curve generation. Intended for research use only, the assay serves as a reproducible tool for measuring circulating and secreted Nidogen-1 and pairs well with immunohistochemistry and western blot workflows that profile basement membrane components in tissue and cell models.