Human Nidogen-1/Entactin ELISA Kit

From Assay Genie, this Sandwich ELISA (Double Antibody) kit is designed for the sensitive and accurate quantitative measurement of Nidogen-1 (Entactin) levels in human samples. It is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Nidogen-1 is described as a key component of the extracellular matrix that plays a vital role in cell adhesion, migration, and tissue development, with its dysregulation implicated in cancer, cardiovascular disorders, and neurological conditions, supporting its use as a research biomarker. The kit should be stored at 2–8°C for 12 months.

Reported linearity across 1:2, 1:4, and 1:8 dilutions spans 85–104% for serum, 82–101% for EDTA plasma, and 80–97% for heparin plasma. Recovery is reported as 98–103% for serum (average 100%), 91–101% for EDTA plasma (average 96%), and 87–103% for heparin plasma (average 93%). The protocol uses a biotin-labeled detection antibody with HRP-Streptavidin (SABC), TMB substrate for color development in the dark, and absorbance readings at 450 nm. Associated UniProt entry: P14543.