Human NID2/Nidogen-2 ELISA Kit

A high-quality Sandwich ELISA kit from Assay Genie designed for the accurate quantification of Nidogen-2 levels in human samples. The assay uses a colorimetric double-antibody sandwich format and is suitable for serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples.

Nidogen-2 is an important extracellular matrix protein involved in cell-matrix interactions and tissue organization. It participates in processes such as cell adhesion, migration, and differentiation. Dysregulation of Nidogen-2 has been implicated in cancer, autoimmune disorders, and developmental abnormalities, underscoring its significance as a potential biomarker for disease research.

The assay procedure involves primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding for 60 minutes, HRP-Streptavidin binding for 30 minutes, TMB substrate development in the dark for 10–20 minutes, and absorbance measurement at 450 nm. Linearity has been validated in serum, EDTA plasma, and heparin plasma at serial dilutions of 1:2, 1:4, and 1:8. Recovery ranges are 86–104% in serum (average 93%), 87–100% in EDTA plasma (average 91%), and 90–104% in heparin plasma (average 96%). The kit includes a pre-coated microplate, lyophilized standard, concentrated biotin-labeled antibody, HRP-Streptavidin conjugate, TMB substrate, dilution buffers, wash buffer, and stop solution.