Human Nicotinamide phosphoribosyltransferase (NAMPT) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit enables quantitative detection of Human Nicotinamide Phosphoribosyltransferase (NAMPT), also known by the synonyms Pre-B-cell colony-enhancing factor 1, Visfatin, Pre-B cell-enhancing factor, NAmPRTase, PBEF, and PBEF1. The assay is reactive with human samples, including serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids, and is specific for both natural and recombinant human Nicotinamide Phosphoribosyltransferase.

Manufacturer-reported performance figures include a detection range of 1.56-100ng/mL and a sensitivity of 0.78ng/mL. Precision is reported as an intra-assay CV of 4.9% and an inter-assay CV of 6.4%. Linearity across dilutions of 1:2 to 1:16 is 98-109% in serum (N=5), 92-115% in EDTA plasma (N=5), and 98-117% in heparin plasma (N=5). Recovery averages 80% (range 80-86%) in serum and 82% (range 80-88%) in plasma.

NAMPT (UniProt P43490) is the rate-limiting component of the mammalian NAD biosynthesis pathway, catalysing the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide. The protein functions as a homodimer and is localised to the nucleus, cytoplasm, and as a secreted form. The kit is classified for research use only and is grouped within the immunology research area.