Human Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) ELISA Kit

NOX4 is a constitutively active NADPH oxidase that primarily generates hydrogen peroxide rather than superoxide, distinguishing it from the inducible NOX2 complex. The enzyme governs redox signaling, transforming growth factor-beta driven fibrosis, and metabolic stress responses across renal, cardiac, and hepatic tissues.

This microplate-based kit from Assay Genie employs a two-antibody capture immunoassay to quantify NOX4 protein in tissue homogenates, clarified cell lysates, and plasma fractions. Each package supplies pre-coated wells, chromogenic substrate, concentrated assay diluent, wash buffer, stop solution, and a recombinant NOX4 standard sufficient for ninety-six determinations.

Standard workflow loads 50 µL of prepared sample per well alongside serially diluted standards. Following incubation and chromogen development, absorbance readings are captured on a standard plate reader and concentrations interpolated from the generated standard curve. Unopened kits should be stored refrigerated; reconstituted reagents benefit from aliquoting and freezing at lower temperatures to limit freeze-thaw degradation.

Researchers apply this assay to investigate oxidative stress within chronic kidney disease models, pulmonary fibrosis progression, vascular remodeling, and hepatocellular injury, where relative NOX4 expression helps stratify disease severity and monitor therapeutic response.