Human NFE2L1/Nuclear factor erythroid 2-related factor 1 ELISA Kit

A Sandwich ELISA from Assay Genie configured for the quantitative detection of human NFE2L1 (Nuclear factor erythroid 2-related factor 1). The assay uses a double-antibody, colorimetric format and is validated for use with human serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples. The kit falls within the metabolism research area and is associated with UniProt entry Q14494.

Reported analytical performance includes a sensitivity of 46.875 pg/mL and a working range of 78.125–5000 pg/mL. Linearity assessed at 1:2, 1:4 and 1:8 dilutions yielded values of 87–102%, 87–102% and 86–105% for serum; 88–101%, 83–89% and 82–97% for EDTA plasma; and 80–97%, 88–95% and 83–98% for heparin plasma. Spike-and-recovery across the same matrices averaged 95% (range 85–100%) for serum, 95% (range 86–103%) for EDTA plasma and 96% (range 85–104%) for heparin plasma. Kit components are stored at 2–8°C for 12 months, with the colorimetric endpoint read at 450 nm.

Alternative names for the target protein include NF-E2-related factor 1, NFE2-related factor 1, Transcription factor LCR-F1, Transcription factor HBZ17, Transcription factor 11, TCF-11, and Locus control region-factor 1.