Human NFATC2 ELISA Kit (HUFI07520)

This microplate immunoassay from Assay Genie supports quantitative measurement of Nuclear Factor of Activated T-Cells, Cytoplasmic 2 (NFATC2) protein in human research specimens. NFATC2 functions as a calcium-dependent transcription factor central to T-cell receptor signaling, where calcineurin-mediated dephosphorylation triggers nuclear translocation and downstream cytokine gene expression, including IL-2, IL-4, and TNF-alpha. Investigators examining immune cell activation pathways, transplant pharmacology, calcineurin inhibitor mechanisms, or NFAT-family contributions to lymphocyte differentiation apply this reagent in their work. The 96 Assays configuration supplies wells for standard curves and replicate determinations, with pre-coated plates, biotinylated detection antibody, streptavidin conjugate, and lyophilized standards included in the kit. The ELISA procedure yields an absorbance-based readout proportional to NFATC2 concentration in each well, allowing interpolation against the supplied reference preparation. Each plate accommodates multiple sample dilutions, supporting investigation of signaling pathway dynamics in cell culture supernatants, tissue homogenates, and human-derived material collected under appropriate ethics oversight. For research use only; not intended for diagnostic or therapeutic procedures.