Human NF-κB p65/Nuclear Factor Kappa B p65 ELISA Kit

A Sandwich ELISA kit from Assay Genie for the quantitative measurement of human NF-κB p65 (Nuclear Factor Kappa B p65) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The double-antibody sandwich format uses a biotin-labelled detection antibody and HRP-Streptavidin conjugate, with TMB substrate for colorimetric readout measured at 450 nm.

Reported performance characteristics include a sensitivity of 0.188 ng/mL and a detection range of 0.313-20 ng/mL. NF-κB p65 is a transcription factor implicated in immune response, inflammation, and cell survival, with dysregulated signalling linked in the literature to cancer, autoimmune disorders, and inflammatory conditions. The associated Uniprot accession is Q04206.

Reagents supplied include a pre-coated microplate, lyophilized standard, biotin-labelled antibody, HRP-Streptavidin conjugate, TMB substrate, dilution and stop solutions, and wash buffer. Reagents should be stored at 2-8°C, with the kit stable for 12 months under these conditions. The procedure involves sequential incubations with sample, biotinylated antibody, and SABC, followed by TMB colour development and absorbance reading at 450 nm.