Human Neurotensin Receptor 1,High Affinity (NTSR1) ELISA Kit

The Assay Genie immunoassay enables quantitative measurement of Neurotensin Receptor 1 (NTSR1), a G protein-coupled receptor that mediates neurotensin signaling in the central nervous system and peripheral tissues. Researchers studying GPCR pharmacology, neuropeptide signal transduction, and receptor expression dynamics use this reagent to profile NTSR1 levels in tissue homogenates and cell lysates prepared from human-derived samples. The plate-based format supplies all reagents needed to generate a standard curve and run experimental samples in parallel, supporting batch analysis of multiple conditions or dose-response studies. Each kit is configured for 96 assays, making it practical for screening compound libraries or comparing receptor abundance across matched patient cohorts.

NTSR1 is of interest across several research areas, including investigations of dopaminergic circuitry, neuropathic pain pathways, and the role of neurotensin signaling in oncological contexts such as colorectal, prostate, and small cell lung carcinoma models. The receptor couples primarily through Gq/11 to mobilize intracellular calcium and activates downstream effectors including phospholipase C, linking ligand binding to downstream transcriptional changes. The kit supports research use only workflows and is intended for laboratory professionals characterizing receptor abundance in crude membrane preparations or whole-cell lysates. Reagents should be stored refrigerated at 4°C, with standard laboratory practice of allowing all components to equilibrate to room temperature before use to ensure consistent binding kinetics across the plate.