Human Neurotensin PharmaGenie ELISA Kit (SBRS0099)

Beyond standard curve generation, this Assay Genie kit is validated for cross-reactivity with neurotensin precursor fragments, allowing researchers to distinguish the mature 13-amino acid peptide from processing intermediates in tissue homogenates and cerebrospinal fluid. The pre-coated microplate uses 8 x 12 removable strips, and the protocol accommodates duplicate wells for standards, blanks, and unknowns. Reconstituted lyophilized standards remain stable for up to 30 days at -20 °C when aliquoted to limit freeze-thaw cycles. Published reports cite the assay in investigations of NTSR1 and NTSR2 G-protein coupled receptor signaling, dopamine D2 receptor interactions in the nucleus accumbens, and gut-brain peptide release following meal challenges. Performance characteristics include intra-assay CV below 10% and inter-assay CV below 12% across the calibrated range, with spike recovery typically between 85 and 115 percent in serum and EDTA plasma matrices. For in vitro research use only.