Human Neuropeptide S ELISA Kit

Designed for the quantitative measurement of human neuropeptide S, this colorimetric ELISA kit from Assay Genie uses a sandwich-based double-antibody method to deliver high sensitivity and specificity. The assay is intended for use with research samples and accepts a broad range of human specimen types, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Detection is performed by measuring absorbance at 450 nm after TMB substrate development.

Key analytical specifications include a sensitivity of 46.875 pg/mL and a working range of 78.125–5000 pg/mL. Reported linearity values fall between 80% and 105% across serum, EDTA plasma, and heparin plasma tested at 1:2, 1:4, and 1:8 dilutions, while recovery averages range from 92% to 99% for the same matrices. The assay procedure follows a six-step workflow: reagent equilibration and plate setup; primary incubation at 37 °C for 90 minutes; biotin-labelled detection antibody incubation for 60 minutes; HRP-streptavidin binding for 30 minutes; TMB colour development in the dark for 10–20 minutes; and a stop reaction with absorbance reading at 450 nm. Storage is recommended at 2–8 °C for 12 months.

Neuropeptide S is a central nervous system peptide implicated in the regulation of stress response, anxiety, and fear-related behaviours, and its involvement in neuropsychiatric conditions such as anxiety disorders and PTSD makes it a valuable biomarker for mental health research and drug development. The kit includes a pre-coated dismountable microplate, lyophilised standard, biotin-labelled antibody (100×), HRP-streptavidin conjugate (100×), TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, 25× wash buffer, plate sealers, and a technical manual.