Human Neuropeptide FF receptor 2 (NPFFR2) ELISA Kit

Manufactured by Assay Genie, this ELISA kit is designed for the detection of human Neuropeptide FF receptor 2 (NPFFR2) in serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids. NPFFR2 is a G-protein coupled receptor that mediates signaling by NPAF and NPFF neuropeptides, also known as morphine-modulating peptides, and has been implicated in pain perception, stress response, and mood regulation.

Key performance specifications include a detection range of 0.625-40ng/mL, a sensitivity of 0.22ng/mL, an intra-assay CV of 5.6%, and an inter-assay CV of 9.2%. The kit shows specificity for natural and recombinant human NPFFR2 (UniProt Q9Y5X5) and is intended for research use only within the Neurosciences research area. Linearity has been validated across dilutions in serum, EDTA plasma, and heparin plasma, with recovery ranging from 83-95% in serum and 85-97% in plasma.

NPFFR2 is a multi-pass membrane protein localized to the cell membrane and belongs to the G-protein coupled receptor 1 family, with four isoforms produced by alternative splicing. The gene is located at chromosomal position 4q21, and the encoded protein may function in pain modulation and regulation of the opioid system.