Human Neuropeptide FF (NPFF) ELISA Kit

Quantitative Neuropeptide FF (NPFF) measurement supports research into pain modulation, opioid tolerance, and thermoregulation pathways. The Assay Genie competitive ELISA Kit produces absorbance-based readouts readable on standard plate readers, with each kit supplied as a complete package containing a pre-coated microplate, detection reagents, lyophilized standards, and assay buffers sufficient for one full microplate run.

Validated for human sample reactivity, the kit accommodates multiple specimen types relevant to neuropeptide biology, including cell culture supernatants from neuronal lines, cell lysates from receptor-transfected models, and tissue homogenates from discrete brain regions. This specimen flexibility bridges in vitro receptor pharmacology with in vivo neuropeptide release studies.

Applications span GPR147 (NPFFR1) and GPR74 (NPFFR2) receptor signaling investigations, hypothalamic-pituitary-adrenal axis research, and inflammatory pain pathway studies. The microplate configuration handles batch sample processing for medium-throughput screening workflows.

For research use only. Reagents require refrigerated storage between runs; substrate solutions should be protected from light to preserve enzymatic activity across the working shelf life.