Human Neurofilament, Light Polypeptide (NEFL) ELISA Kit

NEFL, also known as neurofilament light chain (NfL), is a cytoskeletal intermediate filament protein of approximately 68 kDa expressed in myelinated axons, and circulating concentrations of this biomarker are widely used in studies of neuroaxonal injury in multiple sclerosis, Alzheimer's disease, amyotrophic lateral sclerosis, and traumatic brain injury. The Human Neurofilament, Light Polypeptide (NEFL) ELISA Kit from Assay Genie provides a colorimetric immunoassay for the quantitative measurement of NEFL in human research samples.

Each kit supplies pre-coated microplate strip wells, a calibrator panel for constructing a standard curve via serial dilution, enzyme-conjugated detection antibody, wash buffer, substrate, and stop solution, with materials sufficient for 96 assays. The microplate format allows partial-plate runs when sample throughput is limited, and duplicate wells are typically used alongside kit controls to track inter- and intra-assay reproducibility.

Validated sample types for this research-use-only kit include serum-derived material, plasma-derived material, and cerebrospinal fluid (CSF). Recommended handling involves centrifugation of collected samples to clear particulates, aliquoting to limit freeze-thaw cycles, and storage of aliquots at low temperature until assay setup, with reconstituted reagents kept refrigerated between runs. The protocol supports longitudinal biomarker studies, neuroinflammation research, CSF biomarker discovery, and translational neurology workflows comparing affected cohorts against healthy controls.