Human Nestin/MMEL1 PharmaGenie ELISA Kit (SBRS0918)

Built for neuroscience and stem cell research laboratories, this quantitative immunoassay from Assay Genie simultaneously profiles two human biomarkers connected to neural progenitor identity and metalloprotease activity: Nestin (a class VI intermediate filament protein expressed in neural stem cells and glioblastoma progenitor populations) and MMEL1 (membrane metalloendopeptidase-like 1, a zinc metalloprotease also known as NL1 or NEP2 that participates in neuropeptide processing). Each kit runs 96 assays on a single precoated microplate with absorbance-based readout on standard plate readers. The validated sample matrix spans conditioned media from cultured cells and blood-derived specimens prepared as either plasma or serum, supporting both in vitro work and translational research. Typical applications include monitoring Nestin expression during neural differentiation protocols, comparing MMEL1 levels across tumor-derived cell lines, and screening patient samples in neuro-oncology pipelines. All workflow reagents (precoated plate, detection antibody, recombinant standards, and assay diluents) are provided in the kit. Store components refrigerated between runs to preserve reagent activity and standard curve integrity.