Human Nesfatin-1 (NEFA) ELISA Kit

From Assay Genie, this ELISA kit is designed for the precise measurement of Nesfatin-1 levels in human serum, plasma, and cell culture supernatants. Nesfatin-1, also known as nucleobindin-2 (NUCB2), is described as a peptide hormone that plays a crucial role in regulating food intake, body weight, and energy balance, with studies also linking it to stress responses and metabolic disorders.

Running as a sandwich ELISA with colorimetric detection, the kit is specific for natural and recombinant human Nesfatin-1 and accepts serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Reported performance includes a detection range of 0.312-20ng/mL, a sensitivity of 0.12ng/mL, an intra-assay CV of 6.2%, and an inter-assay CV of 8.5%. Dilutional linearity across 1:2 to 1:16 for serum ranged from 108-118% to 112-120%, for EDTA plasma from 96-107% to 95-104%, and for heparin plasma from 82-92% to 103-113%. Average recovery is 93% (range 87-99%) in serum and 95% (range 89-101%) in plasma.

For research use only, the kit supports investigation of obesity, diabetes, and eating disorders and is listed under the cancer research area, offering a tool for advancing research in metabolism and endocrinology.