Human Nerve Growth Factor (NGF) ELISA Kit

The Human Nerve Growth Factor (NGF) ELISA Kit from Assay Genie provides quantitative measurement of NGF, a neurotrophin critical for the survival of sympathetic and sensory neurons and central to pain signaling, neuroinflammation, and cholinergic neuron maintenance. The microplate assay employs a matched antibody pair specific for human NGF and accepts diverse human sample matrices including tissue homogenates, cell lysates, and cell culture supernatant.

With a detection range of 15.6 to 1000 pg/mL and a minimum detectable dose (sensitivity) below 5 pg/mL, the kit supports research into Alzheimer's disease, chronic pain syndromes, diabetic neuropathy, and neurotrophic factor signaling. Each well requires a 100 µL sample volume, and the standard protocol is typically completed within 3 to 4 hours from sample addition to final readout.

Unopened components should be refrigerated until use, while reconstituted standards and detection antibody vials are stored frozen in single-use aliquots to limit freeze-thaw cycles. The kit is intended for research use only and supports both in vitro cell culture studies and in vivo animal model work on peripheral and central nervous system function.