Human Neprilysin (NEP) ELISA Kit

Quantitative measurement of human neprilysin, a zinc metalloendopeptidase that hydrolyzes vasoactive peptides and amyloid-beta species, is performed on this microplate-based immunoassay from Assay Genie. Investigators in cardiovascular, neurodegenerative, and renal fields track circulating NEP concentrations in liquid human samples to study enzyme shedding and disease progression.

The kit supplies pre-coated microplate wells with anti-NEP capture antibody, biotinylated detector, streptavidin-HRP conjugate, recombinant standard, wash buffer, chromogenic substrate, and stop solution. Signal develops during a standard incubation sequence and is read on a conventional plate reader at the appropriate wavelength. Each plate yields 96 readings, and the protocol can be completed within a standard laboratory workday.

Unopened reagents are stored at 4 degrees C, while reconstituted standards should be aliquoted to minimize freeze-thaw cycles. The 96-assay format fits standard plate-reader workflows, and the kit is intended for research use only.