Human NEK3 ELISA Kit (HUFI07516)

This Assay Genie product delivers quantitative measurement of NIMA-related kinase 3, a serine/threonine protein kinase central to cell cycle regulation at the G1/S transition. NEK3 governs primary cilium dynamics and prolactin-mediated signaling in mammary epithelial cells, and it has been examined in breast cancer progression, neuronal differentiation, and mitotic checkpoint control studies. The microplate-based workflow accommodates full standard curves alongside replicate sample wells in one run, with reagents supplied in vials for single-use aliquots to preserve antibody activity. Storage at 4°C refrigerated is recommended for kit stability, and the human-specific capture antibody allows accurate quantification without significant cross-reactivity with homologous kinases. This in vitro assay can be paired with PCR, western blot, or immunohistochemistry workflows to validate NEK3 expression across cell culture and disease model systems.