Human NEF3  (Neurofilament 3) ELISA Kit (HUFI03400)

The Human NEF3 (Neurofilament 3) ELISA Kit from Assay Genie is intended for in vitro research use only and provides a quantitative tool for measuring neurofilament 3 protein levels in human samples. Neurofilament 3, also known as NF-M, is a cytoskeletal intermediate filament protein expressed in mature neurons, and its release into circulation reflects axonal injury.

This kit is widely applied in neurological research, including studies of amyotrophic lateral sclerosis, multiple sclerosis, Alzheimer's disease, Parkinson's disease, and traumatic brain injury, where NEF3 serves as a biomarker of neurodegeneration and neuronal damage. Researchers also use NEF3 measurements to monitor disease progression and to evaluate therapeutic interventions in preclinical studies.

The microplate-based format supports a high-throughput workflow, with pre-coated capture antibodies specific for the human neurofilament medium chain. Reagents should be stored according to the package insert to maintain activity, and samples should be aliquoted to limit repeated freeze-thaw cycles. The kit is intended for research use only and is not for diagnostic use.