Human NEDD4 ELISA Kit (HUFI07524)

The NEDD4 gene on human chromosome 15q21.3 encodes a HECT-domain E3 ubiquitin ligase that drives ubiquitin-dependent turnover of substrates including PTEN, SGK1, and several voltage-gated sodium channels. Dysregulated NEDD4 activity has been linked to prostate and colorectal cancer progression, Liddle syndrome through ENaC channel overactivity, and defective viral particle release.

This quantitative immunoassay from Assay Genie is configured for measuring endogenous human NEDD4 protein in serum or plasma specimens, supporting translational research into ubiquitination pathways, ion channel regulation, and tumor suppressor biology. The kit ships with all reagents required for a complete assay run and is intended for research use only, not diagnostic procedures.

Researchers commonly apply this kit to compare NEDD4 expression between patient cohorts and matched healthy controls, to validate RNAi or CRISPR knockdown efficiency in cultured cell lines, and to monitor changes following pharmacological inhibition of upstream signaling. Each kit provides reagents sufficient for 96 separate determinations performed on a standard microplate reader.

Before first use, store the sealed kit refrigerated and protect from light. Bring all reagents to room temperature before initiating the assay. Reconstituted standards and detection antibody should be aliquoted to limit freeze-thaw cycles, preserving long-term reactivity.